We used high quality IDT oPools to develop an improved method for the generation of variant libraries, called Scalable and Uniform Nicking (SUNi) mutagenesis. SUNi combines massive scalability with high uniformity to enable cost-effective MAVEs (Multiplexed Assays of Variant Effects) of gene families and eventually genomes. We chose to synthesize our primers as IDT oPools for the quality and higher scale available, picomole-scale yield. This allowed us to maintain the scalability advantage of oligo pools, avoiding amplification which can introduce bias, and maximizing library quality
Taylor L. Mighell
EMBO Postdoctoral Fellow
Centre for Genomic Regulation
RUO24-3146_001
